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HTF and HTF HEPES with HSA - IVF Basics®

IVF Basics® HTF and HTF HEPES with HSA are ready-to-use media and have been developed for washing, handling, conditioning and culturing of human gametes and embryos.

IVF Basics® HTF 0.4% HSA should be pre-equilibrated overnight or at least for a minimum of 4 hours before use at 37ºC and 5% CO2.
IVF Basics® HTF HEPES 0.4% HSA contains HEPES, no CO2 incubation is required.

 

Features & Benefits:

Ready-to-use
Contains Gentamicin
Bicarbonate buffered
HEPES buffered to maintain a stable pH during procedures outside a CO2 incubator (HTF HEPES w/ HSA)
Phenol red for visual pH control (HTF w/ HSA)
Storage at 2oC to 8oC
500ml bottle
1,5 year shelf life

 

Composition:

IVF Basics® HTF and IVF Basics® HTF HEPES with 0.4% HSA consist of a balanced physiologic salt solution, supplemented with carbohydrate energy sources such as glucose, pyruvate and lactate, and 10mg/liter gentamicin sulphate (medicinal substance). IVF Basics® HTF 0.4% HSA is bicarbonate buffered while IVF Basics® HTF HEPES 0.4% HSA additionally contains HEPES.

IVF Basics® HTF 0.4% HSA and IVF Basics® HTF HEPES 0.4% HSA are presupplemented with 4g/liter HSA (medicinal substance derived from human blood plasma) and are ready-to-use.


Quality control:

  • pH: 7,20 – 7,60 (At 5% CO2)
  • Osmolality: 270 - 290 mOsm/kg
  • Sterility test by the No growth
    current pH. Eur.
    2.6.1 / USP <71>
  • Endotoxin: < 0,25 EU/ml
  • Mouse Embryo  ≥ 80%
    Assay:
  • Human sperm ≥ 80%
    survival assay (HSSA)
    (24h exposure): 
  • Certificate of Analysis available upon request.

 

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Specifications: 

IVF Basics® HTF with HSA, Ready-to-use

Catalogue No.

Bottle Size

HTF-B-0,4A-10

10 ml

HTF-B-0,4A-30

30 ml

HTF-B-0,4A-60

60 ml

HTF-B-0,4A-120

120 ml

HTF-B-0,4A-250

250ml

HTF-B-0,4A-500

500 ml



IVF Basics® HTF HEPES with HSA, Ready-to-use

Catalogue No.

Bottle Size

HTF-H-0,4A-10

10 ml

HTF-H-0,4A-30

30 ml

HTF-H-0,4A-60

60 ml

HTF-H-0,4A-120

120 ml

HTF-H-0,4A-250

250ml

HTF-H-0,4A-500

500 ml


 


Instructions For Use 

Summary of Safety and Clinical Performance (SSCP)

Material Safety Data Sheet (MSDS)